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KMID : 1377020140110020171
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Tissue Engineering and Regenerative Medicine
2014 Volume.11 No. 2 p.171 ~ p.177
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Effect of conditioned media collected from human amniotic fluid-derived stem cells (hAFSCs) on skin regeneration and photo-aging
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Huh Man-Il
Kim Min-So
Kim Hong-Kyun
Lim Jeong-Ok
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Abstract
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The purpose of this study is to evaluate the effect of conditioned media (CM) collected from human amniotic fluid-derived stem cells (hAFSCs) on in vitro wound healing and on expression change of MMP-1 and procollagen 1A by ultra-violet A (UVA) irradiation. The conditioned media (CM) were collected from hAFSCs cultured with DMEM/F-12 serum free media. AFSC-CM were tested using in vitro wound healing model, and the results demonstrated that AFSC-CM facilitated cell proliferation in the dermal fibroblast (132.72 ± 7.48%) and skin epidermal cell (125.95 ± 4.61%), and accelerated cell migration after wound was generated. In order to evaluate the effect of AFSC-CM treatment on gene expressions changed by UVA irradiation, AFSC-CM and CM collected from culture of epidermal cell after UVA irradiation were used in dermal fibroblast culture. qRT-PCR data indicated that upregulated MMP-1 expression by UVA was down-regulated and down-regulated procollagen 1A expression was recovered by treatment with AFSC-CM. Collectively, the results suggest that hAFSC-CM has a potential to improve skin regeneration which was damaged by photo-aging.
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KEYWORD
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amniotic fluid-derived stem cell, conditioned media, skin, MMP-1, procollagen 1A
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